TABLE 1.
Peripheral blood counts in control IgG-treated and RB6-treated micea
| Mouse strain | Treatment | Day | % of total counts
|
|||
|---|---|---|---|---|---|---|
| Neutrophils | Lymphocytes | Monocytes | Eosinophils | |||
| C3H | IgG | 0 | 32.2 | 25.7 | 41.9 | 0.7 |
| RB6 | 0 | 1.0 | 42.5 | 56.0 | 0.5 | |
| DBA | IgG | 0 | 23.1 | 23.9 | 53.0 | 0.0 |
| RB6 | 0 | 0.0 | 30.7 | 68.9 | 0.4 | |
| C3H | IgG | 7 | 24.1 ± 7.6 | 46.7 ± 6.0 | 27.8 ± 1.9 | 1.3 ± 0.7 |
| RB6 | 7 | 4.6 ± 3.6 | 44.3 ± 9.9 | 51.2 ± 6.7 | 0.0 ± 0.0 | |
| DBA | IgG | 7 | 25.0 ± 9.4 | 57.1 ± 5.2 | 17.2 ± 5.2 | 0.4 ± 0.6 |
| RB6 | 7 | 0.9 ± 1.3 | 49.2 ± 2.4 | 46.1 ± 1.9 | 0.0 ± 0.0 | |
| C3H | IgG | 14 | 36.0 | 30.7 | 32.9 | 0.4 |
| RB6 | 14 | 0.0 | 44.4 | 55.3 | 0.3 | |
| DBA | IgG | 14 | 27.8 | 38.7 | 33.2 | 0.3 |
| RB6 | 14 | 0.4 | 37.5 | 61.7 | 0.4 | |
| C3H | IgG | 21 | 50.9 ± 0.1 | 22.8 ± 7.0 | 25.4 ± 7.4 | 1.0 ± 0.2 |
| RB6 | 21 | 5.8 ± 0.6 | 48.6 ± 4.0 | 44.9 ± 4.9 | 0.8 ± 0.9 | |
| DBA | IgG | 21 | 33.7 ± 6.0 | 36.6 ± 2.1 | 31.3 ± 8.0 | 0.3 ± 0.2 |
| RB6 | 21 | 6.6 ± 3.9 | 61.4 ± 4.0 | 30.0 ± 1.6 | 2.1 ± 0.5 | |
a
Mice were given daily intraperitoneal injections of 200 μg of control IgG or RB6 beginning on day −1. Mice were infected with 2.5 × 105 B. burgdorferi cells on day 0 and blood was collected from the retroorbital plexus weekly. Blood smears were stained with Diff-Quick, and at least 200 cells per slide were counted. On days 0 and 14 one mouse per treatment per mouse strain was randomly chosen for bleeding. On days 7 and 21 groups of three mice were bled, and the data are averages ± standard deviations. The data are representative of at least four separate experiments.