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. 2004 Sep;72(9):5019–5026. doi: 10.1128/IAI.72.9.5019-5026.2004

FIG. 7.

FIG. 7.

IL-17 induces phosphorylation of ERK 1/2 MAP kinase and IL-8 transcripts in isolated gastric epithelial cells from patients without H. pylori infection. Gastric epithelial cells were stimulated with IL-17 (1 ng/ml) for 0, 15, 30, and 60 min. (A) Western blot analysis of phosphorylated (top blot) and total (bottom blot) ERK 1/2 MAP kinase is shown. (B) RNA transcripts for IL-8 and β-actin were analyzed by RT-PCR at 0 and 60 min. An agarose gel shows RT-PCR products for IL-8 (top) and β-actin (bottom). Examples are representative of four separate experiments using epithelial cells isolated from four patients not infected with H. pylori.