TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Descriptiona | Reference or source |
|---|---|---|
| Strains | ||
| Yersinia spp. | ||
| WA-314 | Y. enterocolitica serotype O:8; clinical isolate; pYVO8+ | 32 |
| WA-C | Plasmidless derivative of WA-314 | 32 |
| WA-C(pYV::CM) | WA-C harboring pYV::CM | This study |
| WA-C(pYVΔH) | WA-C harboring pYVΔH | This study |
| WA-C(pYVΔH+H) | WA-C harboring pYVΔH and pYopH | This study |
| WA-C(pYVΔO) | WA-C harboring pYVΔO | This study |
| WA-C(pYVΔP) | WA-C harboring pYVΔP | This study |
| WA-C(pYVΔE) | WA-C harboring pYVΔE | This study |
| WA-C(pYVΔM) | WA-C harboring pYVΔM | This study |
| WA-C(pYVΔT) | WA-C harboring pYVΔT | This study |
| WA-C(pYVΔQ) | WA-C harboring pYVΔQ | This study |
| E. coli | ||
| DH5α | endA1 hsdR17 (rK− mK+) supE44 thi-1 recA1 gyrA (Nalr) relA1 Δ(lacZYA-argF)U169 (φ80lacZΔM15) | 30 |
| S17-1λpir | pir+tra+ Kanr | 41 |
| Plasmids | ||
| pACYC184 | Low-copy vector; Cmr Tcr | New England Biolabs |
| pACYC177 | Low-copy vector; Kanr Ampr | New England Biolabs |
| pGPCAT | Suicide vector; R6K replicon; Cmr Mob+ | 48 |
| pUC4k | Kanr cassette | Pharmacia-LKB |
| pYV::CM | pYV plasmid harboring Cmr gene from pACYC184 in noncoding region adjacent to yadA | This study |
| pYVΔH | pYV plasmid with yopH replaced by Kmr gene from pACYC177 | This study |
| pYVΔO | pYV plasmid with yopO replaced by Kmr from pACYC177 | This study |
| pYVΔP | pYV plasmid with yopP replaced by Kmr from pACYC177 | This study |
| pYVΔE | pYV plasmid with yopE replaced by Kmr from pACYC177 | This study |
| pYVΔM | pYV plasmid with yopM harboring Kmr from pUC4k | This study |
| pYVΔT | pYV plasmid with yopT replaced by Kmr from pACYC177 | This study |
| pYopHSycH | pACYC184 harboring yopH and sycH | 57 |
a
Ampr, ampicillin resistance; Tcr, tetracycline resistance.