FIG. 2.
Expression of phoPKIM in trans promotes the survival and growth of YPIII(p−) in macrophages. J774A.1 macrophages were infected with YPIII(p−)/MMB67EH (gray bar) or YPIII(p−)/PhoPKIM (solid bar) at an MOI of 5 in the presence of IPTG. The numbers of CFU recovered from the infected macrophages after 0.25 and 24 h of infection were determined as described in Materials and Methods. The percent increase in CFU was calculated by using the following formula: (CFU at T24 − CFU at T0.25)/(CFU at T24) × 100, where T24 and T24 are 0.25 and 24 h after infection, respectively. The data are the averages ± standard deviations of values obtained from two independent experiments with duplicate wells.