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Expression of the virB genes during growth of B. abortus in vitro. (A) Activity of a virB4::lacZYA fusion during growth in TSB. OD600, optical density at 600 nm. (B) Western blot showing detection of VirB1 and VirB5 produced by B. abortus 2308 during growth in TSB at different times after inoculation. The positive controls shown at the right are GST-VirB1 and GST-VirB5.
