virB-1644F |
5′-GCTCTAGAGCGGATTCTACCTCACCTACTGC-3′ (XbaI) |
virB2-B4 |
virB-2457R |
5′-TCCCCCGGGCCTCCTTGTCCTGCGTCTCG-3′ (SmaI) |
virB2-B4 |
virB-219F |
5′-CTCGAGTGAAAATCACCGCATACCAC-3′ (XhoI) |
virB1 upstream |
virB-477R |
5′-ATCGATGAGGACAAGGAATGGCACC-3′ (ClaI) |
virB1 upstream |
virB-1154F |
5′-ACGGCGTAGTTGTTTTCTAACCC-3′ |
virB1 downstream |
virB-1562R |
5′-TCTAGACGGTCTGCTTGCTCAATGTCTATTC-3′ (XbaI) |
virB1 downstream |
virB-988F |
5′-CTCGAGGCTGCCCCAGTAAAAAAACGAC-3′ (XhoI) |
virB2 upstream |
virB-1562R |
5′-ATCGATTCGGTCTGCTTGCTCAATGTCTAT-3′ (ClaI) |
virB2 upstream |
virB-1796F |
5′-CCATCGCCATCATCTGGTCCG-3′ |
virB2 downstream |
virB-2458R |
5′-TCTAGATCTCCTTGTCCTGCGTCTCG-3′ (XbaI) |
virB2 downstream |
KSAC707F1 |
5′-ATCGATTAACCAATTCTGATTAGA-3′ (ClaI) |
Kan cassette |
KSAC1638R |
5′-GAATTCCGTTGTGTCTCAAAATCTCTG-3′ (EcoRI) |
Kan cassette |
virB-5063F |
5′-GAATTCTACGAAGCGGTGATGAGCG-3′ (EcoRI) |
GST-VirB5 fusion |
virB-5549R |
5′-CTCGAGGTGTAGATGATGTGTTGCGTG-3′ (XhoI) |
GST-VirB5 fusion |
virB-629F |
5′-GGAATTCTCCCCCAGCAAGAAGTCG-3′ (EcoRI) |
GST-VirB1 fusion |
virB-1330R |
5′-CTCGAGTGGTGTGCGACTGCCTCCTATC-3′ (XhoI) |
GST-VirB1 fusion |
98736F |
5′-CTCGAGGCTGCGATTTCCATTCACTCC-3′ (XhoI) |
virB upstream |
99854R |
5′-GTCGACTCTTGCCGTCATAATAGCCTGAG-3′ (SalI) |
virB upstream |
112902F |
5′-CTGCAGAAAGCCAACCCCTCGC-3′ (PstI) |
virB downstream |
114022R |
5′-TCTAGACACCTGATTCCACGGAGTG-3′ (XbaI) |
virB downstream |
KSAC707F2 |
5′-GTCGACTAACCAATTCTGATTAGA-3′ (SalI) |
Kan cassette |
KSAC1638R2 |
5′-CTGCAGGTTGTGTCTCAAAATCTCTG-3′ (PstI) |
Kan cassette |
SacB-F |
5′-GAGCTCGCTGGCTTAACTATGCGGCA-3′ (XhoI) |
sacB |
SacB-R |
5′-GAGCTCTTCAAACAGGAGGGCTGGAAG-3′ (XhoI) |
sacB |