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. 2004 Sep;72(9):5143–5149. doi: 10.1128/IAI.72.9.5143-5149.2004

TABLE 2.

Primers used in this worka

Primer name Sequence (restriction enzyme) Fragment
virB-1644F 5′-GCTCTAGAGCGGATTCTACCTCACCTACTGC-3′ (XbaI) virB2-B4
virB-2457R 5′-TCCCCCGGGCCTCCTTGTCCTGCGTCTCG-3′ (SmaI) virB2-B4
virB-219F 5′-CTCGAGTGAAAATCACCGCATACCAC-3′ (XhoI) virB1 upstream
virB-477R 5′-ATCGATGAGGACAAGGAATGGCACC-3′ (ClaI) virB1 upstream
virB-1154F 5′-ACGGCGTAGTTGTTTTCTAACCC-3′ virB1 downstream
virB-1562R 5′-TCTAGACGGTCTGCTTGCTCAATGTCTATTC-3′ (XbaI) virB1 downstream
virB-988F 5′-CTCGAGGCTGCCCCAGTAAAAAAACGAC-3′ (XhoI) virB2 upstream
virB-1562R 5′-ATCGATTCGGTCTGCTTGCTCAATGTCTAT-3′ (ClaI) virB2 upstream
virB-1796F 5′-CCATCGCCATCATCTGGTCCG-3′ virB2 downstream
virB-2458R 5′-TCTAGATCTCCTTGTCCTGCGTCTCG-3′ (XbaI) virB2 downstream
KSAC707F1 5′-ATCGATTAACCAATTCTGATTAGA-3′ (ClaI) Kan cassette
KSAC1638R 5′-GAATTCCGTTGTGTCTCAAAATCTCTG-3′ (EcoRI) Kan cassette
virB-5063F 5′-GAATTCTACGAAGCGGTGATGAGCG-3′ (EcoRI) GST-VirB5 fusion
virB-5549R 5′-CTCGAGGTGTAGATGATGTGTTGCGTG-3′ (XhoI) GST-VirB5 fusion
virB-629F 5′-GGAATTCTCCCCCAGCAAGAAGTCG-3′ (EcoRI) GST-VirB1 fusion
virB-1330R 5′-CTCGAGTGGTGTGCGACTGCCTCCTATC-3′ (XhoI) GST-VirB1 fusion
98736F 5′-CTCGAGGCTGCGATTTCCATTCACTCC-3′ (XhoI) virB upstream
99854R 5′-GTCGACTCTTGCCGTCATAATAGCCTGAG-3′ (SalI) virB upstream
112902F 5′-CTGCAGAAAGCCAACCCCTCGC-3′ (PstI) virB downstream
114022R 5′-TCTAGACACCTGATTCCACGGAGTG-3′ (XbaI) virB downstream
KSAC707F2 5′-GTCGACTAACCAATTCTGATTAGA-3′ (SalI) Kan cassette
KSAC1638R2 5′-CTGCAGGTTGTGTCTCAAAATCTCTG-3′ (PstI) Kan cassette
SacB-F 5′-GAGCTCGCTGGCTTAACTATGCGGCA-3′ (XhoI) sacB
SacB-R 5′-GAGCTCTTCAAACAGGAGGGCTGGAAG-3′ (XhoI) sacB
a

Nucleotides in primers are numbered according to position in the B. abortus virB locus as indicated by the sequence found under GenBank accession number AF226278 (30). Primers 98736F, 99854R, 112902F, and 114022R were designed using the B. suis genome sequence (26).