TABLE 3.
The SD1 mutant is sensitive to detergents and hydrogen peroxide at 37°Ca
Bacterial strain | % of growth inhibition on:
|
MIC of H2O2 (mM)d | |
---|---|---|---|
0.5% Deoxycholateb | 0.85% Ox bilec | ||
SD1 | 86 | 98 | 27 |
Wild type | 0 | 0 | 55 |
No significant differences in sensitivity were found when experiments were performed at 28°C.
Salmonella serovar Enteritidis strains were grown to log phase in Trypticase soy broth (TSB), centrifuged, and suspended in physiologic solution. Appropriated dilutions were then placed onto Trypticase soy agar with different concentrations of deoxycholate.
Salmonella serovar Enteritidis strains were grown in TSB and plated on different concentrations of ox bile extracts.
Salmonella serovar Enteritidis strains were grown to log phase in TSB and incubated with hydrogen peroxide for 18 h at 37°C.