Effect of antibiotic treatment on microbiota populations. At various times, murine tissues were harvested, homogenized in sterile water, and plated with the following media to enumerate cecal bacterial populations: VRBA for enteric bacteria, TSA II blood agar in an anaerobic chamber for total anaerobic bacteria, and SDA for yeast. (a) C. albicans tissue deposition at day 1 postinfection. (b) Cecal bacterial populations before and after antibiotic treatment. (c) Cecal bacterial populations at day 12 postinoculation with C. albicans. n = 7 to 8 mice per time point pooled from two separate experiments. Asterisk, P < 0.01; n.d., not detected.