FIG. 5.

Effect of antibiotic therapy and fungal microbiota increase on lung inflammation and goblet cell metaplasia in response to intranasal conidia exposure. Mice were treated as described in the legend to Fig. 1. At day 12 posttreatment, lungs were harvested, fixed, sectioned, and stained with hematoxylin and eosin (H&E) (a to d) or PAS (e to h). In panel d, the arrow highlights the presence of numerous eosinophils in mice treated with antibiotics and colonized with GI C. albicans. In panel f, PAS stains mucus pink, indicating goblet cell metaplasia in the airway epithelium (arrow). Magnifications and groups are indicated on the photomicrographs.