FIG. 10.

Gastric cell migration is significantly inhibited by the addition of CH275 SSTR-1 analog at 1 and 10 nM. NCI-N87 cells were grown to confluence in six-well plates and were serum starved for 24 h prior to the experiment. Primary cells were plated at high density in the absence of significant cell division. A 10-μl pipette tip was used to create a wound across the center of the well. After a washing to remove cell debris, cells were either untreated or treated with 1 or 10 nM CH275, an SSTR-1-specific analog. The rate of wound closure was measured at 12-h intervals over a 48-h period. The rate of migration is expressed as millimeters per 12-h period, and the effect of SSTR-1 analog treatment was assessed at each time point by the unpaired Student t test. Under control conditions in the presence of 5% serum the primary (A) and NCI-N87 (C) cells migrated as an integrated sheet. Cell migration was significantly inhibited by the addition of CH275 SSTR-1 analog at 1 and 10 nM. (B) Primary cells; (D) NCI-N87 cells.*, P = <0.05. The cell line data are from six individual experiments; the primary-cell preparation data are from three individual experiments.