FIG. 5.

H. pylori infection of NCI-N87 cells causes internalization of IQGAP-1. NCI-N87 cells were plated on APES-coated glass coverslips and incubated for 48 h to facilitate attachment prior to infection with H. pylori. Control (Cnt) and infected (+H.p) cells were fixed 48 h postinfection in 4% paraformaldehyde for 10 min at room temperature. (A) Control cells were immunostained with monoclonal anti-IQGAP-1 antibodies for 18 h at 4°C. Note the presence of IR at adherens junctions (arrows). (B) Cells were infected with H. pylori and immunostained for IQGAP-1. Note the dramatic increase in internalized protein in tubulovesicles (arrows). Scale bar = 5 μm. Images are representative of three independent experiments.