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Adherence of M. fermentans strain PG-18 to HeLa cells as a function of time and temperature. Radiolabeled microorganisms were incubated with HeLa cells in the adherence assay mixture for up to 4 h at 37°C (▴), 22°C (▪), or 4°C (•), and binding was determined as described in Materials and Methods. The data are means ± standard deviations of three independent experiments. (Inset) Effect of divalent cations on binding. Symbols: (○), MgCl2; (•), CaCl2.
