TABLE 1.
M. fermentans prepn | Adherence to HeLa cells (cpm/106 HeLa cells) | Invasion of HeLa cells (CFU/106 cells) |
---|---|---|
Native | 2,450 ± 250 | 250 ± 150 |
Pg treated | 9,800 ± 550 | 560 ± 250 |
Pg and uPA treated | 9,200 ± 600 | 4,500 ± 1,100 |
M. fermentans was treated with 25 μg of Pg per ml or with Pg (25 μg/ml) and uPA (100 u/ml) for 30 min at 37°C. HeLa cells were then infected with the M. fermentans preparations at a multiplicity of infection of 100, and invasion was determined after 3 h of incubation at 37°C