Figure 4.

The detection of HSP60 protein expression on SH-SY5Y cells. (a) Western blotting of HSP60 and β-actin from SH-SY5Y cells cultured on different surfaces and/or different medium. The signals were quantified and the data are presented as the means ± SEMs; p < 0.05 or 0.001 indicates statistical significance, as determined by unpaired Student's t-test. (b) Analyses of representative samples of SH-SY5Y cells expression of vimentin and HSP60 are shown. Immunochemical stains for DAPI (blue), vimentin (green), and HSP60 (red) for adhered SH-SY5Y cells on indicated surfaces and/or conditioned media for 24 h (scale bars, 10 μm; confocal microscope, 400x). ^∗ p < 0.05 and ^∗∗∗ p < 0.001.