The plcR tree of the B. cereus group, based on its nucleotide sequences. This tree was constructed by the neighbor-joining method. B. mycoides, which was used as an outgroup in Fig. 1, was not used as an outgroup because it was not distinguished from the ingroup strains. Thus, the midpoint rooting method was applied to root this tree. Clusters in the chromosomal gene tree (Fig. 1) are indicated on the right. Branches supported by more than 50% in the bootstrap analysis (1,000 replications) are indicated.