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Time course of TNF-α-induced IL-8 response. T84 cells were incubated with TNF-α (10 ng/ml) for 0.5, 1, 2, 4, 6, and 12 h. T84 cells constitutively secreted IL-8. TNF-α significantly increased IL-8 secretion starting from 2 h of incubation with T84 cells. **, P < 0.01, compared with media control (n = 3).
