FIG. 6.

S. suis type 2 purified CPS effect on Akt and PKCα phosphorylation levels. Lysates obtained from 15-min-infected cells with encapsulated strain 735, nonencapsulated mutant 2A, or purified CPS (1, 10, or 100 μg/ml) were subjected to Western blotting, and the Akt and PKCα phosphorylation levels were revealed by using anti-Phospho-Akt (P-Akt) or anti-Phospho-PKCα (P-PKCα). Stripped filters were then reprobed with anti-Akt polyclonal antibody or anti-PKCα monoclonal antibody to confirm equal levels of protein. The results are representative of three individual experiments.