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Kinetic cytochrome c assay measuring superoxide production. (A) PMN alone or in the presence of A. phagocytophilum (Ap) or E. coli (Ec) with or without PMA added at time zero; (B) PMN preincubated with A. phagocytophilum for 0, 30, 60, and 120 min. Unbound A. phagocytophilum was removed prior to the start of the cytochrome c assay.
