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Xanthine conversion by xanthine oxidase in a cell-free system, producing O2− and urate. (A) O2− production was measured in the absence of A. phagocytophilum (Ap) (solid diamonds), in the presence of increasing concentrations of A. phagocytophilum, and in the presence of uninfected HL-60 control. (B) Urate production of the same samples at the same times as in panel A.
