Table 1.
Cell Type | Transfection | % cells stained * |
DS2 | pMT/V5-His/lacZ | 82.86 ± 9.12 |
DS2 | pMT/V5-His/lacZ + dsRNA | 17.70 ± 4.25 |
DS2 | pMT/V5-His/lacZ + dsRNA + pMT-HC-Pro | 45.78 ± 4.89 |
DS2 | pMT/V5-His/lacZ + dsRNA + pMT-HC-Pro-K | 14.62 ± 2.81 |
DS2 | pMT/V5-His/lacZ + dsRNA + pMT-2b | 11.13 ± 1.58 |
DS2 | pMT/V5-His/lacZ + dsRNA + pMT-16K | 47.25 ± 3.4 |
DS2-HC-Pro | pMT/V5-His/lacZ | 42.04 ± 3.07 |
DS2-HC-Pro | pMT/V5-His/lacZ + dsRNA | 31.70 ± 2.46 |
DS2-scAb S20 | pMT/V5-His/lacZ | 40.67 ± 2.36 |
DS2-scAb S20 | pMT/V5-His/lacZ + dsRNA | 6.50 ± 1.2 |
DS2-2b | pMT/V5-His/lacZ | 41.16 ± 2.36 |
DS2-2b | pMT/V5-His/lacZ + dsRNA | 9.17 ± 2.56 |
* Four randomly selected fields of view each containing ~100 cells were selected in each of duplicate plates and the number of cells staining blue was counted for each experiment. The figures shown are the means from four experiments for the DS2 cells and three experiments for the DS2-HC-Pro and DS2-ScAb S20 cells.