Table 3. Human DEPDC and internal control genes and their primer sequences information.
| Gene | Primer sequences | Tm(°C) | %GC | Product(bp) |
|---|---|---|---|---|
| DEPDC1-F | GAAGCAGTGGATTGGCTTTATG | 62 | 45.5 | 136 |
| DEPDC1-R | CCCACCTCCCTTTGATATCTTC | 62 | 50.0 | |
| DEPDC1B-F | GGAAATTCTGAAAGTCCCTTTGG | 62 | 43.5 | 98 |
| DEPDC1B-R | CCATATCAGCTCCTGGGTATTT | 62 | 45.5 | |
| DEPDC2-F | GAGCACAAAGCCAAGAGAGA | 62 | 50.0 | 100 |
| DEPDC2-R | TCCTACAGCATGCACAACAG | 62 | 50.0 | |
| DEPDC3-F | GCAGAGAAATGGTGGAACTCT | 62 | 47.6 | 105 |
| DEPDC3-R | CTCCTGGTGCTACAGGAAATAC | 62 | 50.0 | |
| DEPDC4-F | GAACCGTAGAGATGGCTTCTG | 62 | 52.4 | 101 |
| DEPDC4-R | GGGCCTGAAGAGAGTGAATAAT | 62 | 45.5 | |
| DEPDC5-F | CTCCTGTGGCTTCTTGTTAGT | 62 | 50.0 | 106 |
| DEPDC5-R | TGATGTTGAGTGGGATGAAGAG | 62 | 50.0 | |
| DEPDC6-F | TTGTGGTGCGAGGAAGTAAG | 62 | 47.6 | 107 |
| DEPDC6-R | CCGTTGACAGAGACGACAAA | 62 | 45.5 | |
| DEPDC7-F | ACCTTCCACTTCTTGACTCCTTAC | 57.8 | 45.8 | 155 |
| DEPDC7-R | CGAGAGCCACTCATCTTCCTG | 57.5 | 57.1 | |
| β-actin-F | CGTGCGTGACATTAAGGAGAAG | 57.2 | 50.0 | 176 |
| β-actin-R | GGAAGGAAGGCTGGAAGAGTG | 57.5 | 57.1 |
Note: F, forward; R, reverse.