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. 2016 Dec 21;6:39618. doi: 10.1038/srep39618

Figure 6. Time-lapse BRET imaging of cytosolic ATP levels in living HeLa cells.

Figure 6

(A) Sequential pseudocolor images the BRET ratio of HeLa cells stably expressing cyt-BTeam. Intracellular ATP depletion in the cells was monitored after addition of 3 μg/mL oligomycin A and 20 mM 2-deoxyglucose at time = 0 (min). The numbers in the figure represent the time (min) after addition of the inhibitors. The scale bar represents 8 μm. (B) Time-course of the BRET ratio of HeLa cells stably expressing cyt-BTeam. The blue and red lines represent the ratios obtained from control cells and cells treated with inhibitors, respectively. Error bars represent standard deviation (n = 8 individual cells). The arrow indicates the time of addition of the inhibitors to the medium.