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. 2016 Dec 21;6:39548. doi: 10.1038/srep39548

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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A significant increase in the expression level of necroptotic protein in A549 cells (a) whereas increase in apoptotic protein in BEAS-2B cells (c,e) was found after the 24 h exposure of TRGO. (b,d,f) Quantitation was done in Biorad Versa DOC (Bio-Rad Laboratories, Inc. Hercules, USA) with the help of Quantity One Quantitation Software version 4.3.1 and expressed in fold change. Further, in the presence of Nec-1 (g) and Q-Vd-OPh (i) the expression level of respective proteins was found to get attenuated which confirmed the activation of differential cell death pathway upon GD exposure. (h,j) Quantitation was done in Biorad Versa DOC (Bio-Rad Laboratories, Inc. Hercules, USA) with the help of Quantity One Quantitation Software version 4.3.1 and expressed in fold change. Full lengths blots were shown in Fig. S11.