Figure 3. Native PAGE results for RNA oligonucleotides (consisting of residues from 1 to 34 for the pseudoknots and residues 1–19 for the hairpin containing stem 1).

The same constructs were used for the thermal melting studies. The stem 1-loop 2 minor-groove base triple formation is maintained in SF206, SF209, and SF211. The mutants SF217 and SF220 have a single base mutation of a residue in loop 2 involved in base triple interactions.