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. 2004 Oct;72(10):5676–5686. doi: 10.1128/IAI.72.10.5676-5686.2004

FIG. 2.

FIG. 2.

Association of syringed and sonicated (three 30-s bursts) M. tuberculosis, strain Erdman with various macrophage populations was assessed and is expressed as a percentage of the macrophage population that was infected with one or more syringed bacteria (>1 syringed), 10 or more syringed bacteria (>10 syringed), one or more sonicated bacteria (>1 sonicated), and 10 or more sonicated bacteria (>10 sonicated). The mean ± standard error of the mean are shown for (A) murine resident peritoneal macrophages (R-PMΦ), 13 experiments representing a total of 35 replicate coverslips; (B) thioglycolate-elicited peritoneal macrophages (E- PMΦ), four experiments representing a total of eight replicate coverslips; (C) freshly explanted alveolar macrophages at day zero (AMΦ-D0) or in vitro-differentiated alveolar macrophages at day 4 (AMΦ-D4), four experiments representing a total of 12 replicate coverslips; and (D) human monocyte-derived macrophages (MDM) or macrophage-like cells (THP-1), three experiments representing a total of nine replicate coverslips. The MOI was 20 bacteria per macrophage for all macrophage types (A, B, and D) except alveolar macrophages (C), for whichthe MOI was 500:1. *, P < 0.0001, and #, P < 0.05, when comparing sonicated to syringed bacteria.