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. 2016 Dec 21;7:1915. doi: 10.3389/fpls.2016.01915

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Copyright © 2016 Di, Gomila, Ma, van den Burg and Takken.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Avr2 accumulates in xylem sap and apoplastic fluids of Avr2 plants. (A) Western blot showing accumulation of HASBP-tagged Avr2 in total protein extracts of transgenic tomato plants expressing either full-length Avr2 or ΔspAvr2. The top blot was probed with an antibody targeted against Avr2 while the bottom blot was developed using an HA antibody. The top band (^∗) corresponds to the size of HASBP-tagged Avr2 whereas the lower band (#) represents the size of a non-tagged Avr2. (B) Western blot of xylem sap and apoplastic fluid isolated from the above mentioned plants probed with an Avr2 specific antibody. Avr2 accumulates in apoplastic fluid and xylem sap of transgenic tomato plants expressing full-length Avr2-, but not in plants expressingΔspAvr2. The molecular weight, as indicated by the precision plus protein standard (Bio-Rad), is shown on the left.