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. 2016 Dec 21;7:1915. doi: 10.3389/fpls.2016.01915

FIGURE 3.

FIGURE 3

I-2-carrying tomato plants do not trigger immune signaling upon Avr2 exposure. (A) Scions of 4-week-old tomato plants expressing I-2 grafted onto a wild-type Moneymaker, a ΔspAvr2 or an Avr2 rootstock. Representative grafts are shown 4-weeks-post grafting. Note that all grafts grew normally and did not develop autoimmune symptoms (B) Western blot analysis of xylem sap harvested ±10 cm above the graft. The Avr2 protein could be readily detected in xylem sap of I-2 scions placed on an Avr2 rootstock, but not in xylem sap isolated from scions grafted on either wild-type or a ΔspAvr2 roots stock. As a positive reference Avr2-containing xylem sap was harvested from tomato plants inoculated with Fol007. (C) Avr2-7 and ΔspAvr2-30 transformants were crossed to I-2 tomato plants. Two weeks after germination ΔspAvr2/I-2 plants developed clear autoimmune phenotypes; i.e., necrotic lesions, reduced plant weight and stunted growth, whereas no symptoms developed on Moneymaker/I-2 or Avr2/I-2 progeny. (D) Western blot analysis shows accumulation of Avr2 in Avr2 and ΔspAvr2 transgenic tomato plants and their ΔspAvr2/I-2 and Avr2/I-2 progenies. The blot was probed with an antibody targeted against Avr2. Lower panel shows a Ponceau S staining that serves as loading control.