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. 2016 Dec 21;7:1915. doi: 10.3389/fpls.2016.01915

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Copyright © 2016 Di, Gomila, Ma, van den Burg and Takken.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Infiltration of Agrobacterium tumefaciens in Avr2/I-2 tomato plants triggers cell death. (A) Four-week-old wild-type Moneymaker, I-2 and Avr2/I-2 tomato plants infiltrated with either infiltration buffer (“-”) or Agrobacterium expressing GUS or ΔspAvr2. The left side of each leaf is buffer infiltrated and the right site is infiltrated with Agrobacterium carrying either a GUS or ΔspAvr2 construct. Photographs were taken 4 dpi. The bottom panel shows the same leaves stained with trypan blue to visualize cell death. (B) 20 leaves of wild-type Moneymaker, I-2 and Avr2/I-2 tomato plants were scored for their response following infiltration. The assay was repeated twice with similar results.