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. 2016 Dec 21;7:1915. doi: 10.3389/fpls.2016.01915

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Copyright © 2016 Di, Gomila, Ma, van den Burg and Takken.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Verticillium dahliae does not facilitate Avr2 uptake in tomato. (A) Representative pictures of mock (upper row) and V. dahliae race 1 JR2 (bottom row) inoculated Moneymaker, ΔspAvr2 and Avr2 transgenic tomato plants at 21 days post inoculation (dpi). Hyper-susceptibility was only observed in ΔspAvr2 plants. As a measure for disease development (B) fresh weight and leaf canopy surface of inoculated plants was measured. Error bar represent mean ± SE. (^∗P < 0.05; ^∗∗∗P < 0.001). (C) As a measure of V. dahliae colonization stem sections from the cotyledon (top-left), second node (top-right) and fourth node (bottom-center) of each plant were collected at 21 dpi and placed on PDA plates. Pictures were taken 5 days post incubation on plate. (D) Fungal progression in the stem was expressed as a percentage of infected slices.