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. 2016 Dec 21;6:36411. doi: 10.1038/srep36411

Figure 1. Characterization of HSC-like cells in neural-crest derived mandible.

Figure 1

(A) Flow cytometry profiles of CD34ˉLSK cells. Lineage-depleted cells with CD34 negative and c-Kit and Sca-1 double positive selection. (B) Quantitative counts of CD34ˉLSK cells from femur/tibia and mandible at 0.176 ± 0.034 and 0.216 ± 0.027, respectively (mean ± s.d., n = 5). (C) Sorted mandibular CD34ˉLSK cells cultured for 10 days. (D) Proliferation rates of CD34ˉLSK cells from mandible and femur/tibia (mean ± s.d., n = 3). (E) Representative images of colony-forming mandibular CD34ˉLSK cells, including colony-forming unit erythroid (CFU-E), mature burst-forming unit-erythroid (BFU-E), colony-forming unit granulocyte/macrophage (CFU-GM), CFU-granulocyte/ erythroid/ megakaryocyte/ macrophage (CFU-GEMM) and pre-B lymphoid (CFU-Pre B). (F) Colony numbers of mandibular CD34ˉLSK cells (mean ± s.d., n = 3, *p < 0.05). (G) Diagram of LTC-IC assay. (H,I) HSCs cultured on a feeder layer in the myeloid medium for 4 wks and lymphoid medium for 1 wk. (J) Flow cytometry of differentiated long-term culture-initiating cells in methylcellulose culture medium (mean ± s.d., n = 3, **p < 0.01).