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. 2016 Dec 21;6:39130. doi: 10.1038/srep39130

Figure 7. Analysis of the packaging process of the replicated genomic DNA by Southern blot.

Figure 7

(A) total DNA was extracted from the samples (1–9), digested with the restriction enzyme ApaI, and detected by the synthesized DNA probe. The 10 kb ApaI fragment was predicted from the digested concatemeric DNA. The 6.5 kb ApaI fragment was predicted from the digested single viral genome (monomeric DNA). Marker: 15 kb DNA marker. (B) the schematic representation of the predicted phage genomic structures and the corresponding ApaI fragments detected in the Southern blot analysis. α: concatemeric DNA. β: monomeric DNA. The solid purple arrows stood for the terminal direct repeats (TDR) of the phage genome. The red rectangles stood for the DNA probe region.