Table 2. Strains, phage, and plasmids used in this study.
| Strain, phage, and plasmid | Description | Reference |
|---|---|---|
| P. aeruginosa | ||
| PAK | Laboratory strain | 73 |
| RC11-2 | BN889_05221::Tn5G, phage C11 resistant mutant of PAK, Gmr | This study |
| RC11-5 | PA3808::Tn5G, phage C11 resistant mutant of PAK, Gmr | This study |
| RC11-7 | wbpR::Tn5G, phage C11 resistant mutant of PAK, Gmr | This study |
| RC11-10 | PA1993::Tn5G, phage C11 resistant mutant of PAK, Gmr | This study |
| RC11-20 | PA5181::Tn5G, phage C11 resistant mutant of PAK, Gmr | This study |
| RC11-21 | PA0243::Tn5G, phage C11 resistant mutant of PAK, Gmr | This study |
| RC11-22 | PA1115::Tn5G, phage C11 resistant mutant of PAK, Gmr | This study |
| Phage | ||
| C11 | P. aeruginosa lytic phage | 33 |
| Plasmid | ||
| pUCP18 | Broad host range shuttle vector, Apr | 74 |
| pRK2013Tn5G | used for transposon mutagenesis, Gmr | 71 |
| pXL1502 | BN889_05221 gene driven by Plac promoter cloned in pUCP18, Apr | This study |
| pXL1503 | PA3808 gene driven by Plac promoter cloned in pUCP18, Apr | This study |
| pXL1504 | wbpR gene with promoter region cloned in pUCP18, Apr | This study |
| pXL1505 | PA1993 gene with promoter region cloned in pUCP18, Apr | This study |
| pXL1506 | PA5181 gene driven by Plac promoter cloned in pUCP18, Apr | This study |
| pXL1507 | PA0243 gene driven by Plac promoter cloned in pUCP18, Apr | This study |
| pXL1508 | PA1115 gene with promoter region cloned in pUCP18, Apr | This study |
*Apr, ampicillin resistance; Gmr, gentamicin resistance.