Table 3. Primers used in the study.
| Primer | *Primer sequence (5′-3′) | Target region |
|---|---|---|
| OTn1 | GATCCTGGAAAACGGGAAAG | genome DNA regions flanking by Tn5G |
| OTn2 | CCATCTCATCAGAGGGTAGT | |
| BN889_05221-F | CCCAAGCTTGAACGGACGCTCTTTGTG | coding region of gene BN889_05221 |
| BN889_05221-R | CGCGGATCCACACCTTGCGTGAACTGG | |
| PA3808-F | CGCGGATCCGCCAAACAGTTCATCACCACC | coding region of gene PA3808 |
| PA3808-R | CGCGGATCCTTTTCCCAATGCCCGCCCTTCC | |
| wbpR-F | CGCGGATCCCAACAAGCCGCTGAAGCC | gene wbpR with its natural promoter |
| wbpR-R | CGCGGATCCAGAACACCGACGCCCTGG | |
| MFS-F | CGCGGATCCGGCACAACCGATTAGACG | gene PA1993 with its natural promoter |
| MFS-R | CGCGGATCCTACCGACCAGACTCAGGGA | |
| PA5181-F | CGCGGATCCGACAACCTCAACCAGCACT | coding region of gene PA5181 |
| PA5181-R | CGCGGATCCGCCGAGCCCTATTCCTT | |
| PA0243-F | CCCAAGCTTGCGGTGATGCGGTTGGTG | coding region of gene PA0243 |
| PA0243-R | CCCAAGCTTGCGGGAGAGGCAGTCGGT | |
| PA1115-F | CCCAAGCTTGAACGGACGCTCTTTGTG | gene PA1115 with its own promoter |
| PA1115-R | CGCGGATCCACACCTTGCGTGAACTGG | |
| 56-F | GTTGGTTTCTTCCCCGAGG | qPCR primers, coding region of gene ORF056 |
| 56-R | ATTCGTCTGCCTTCCATCGC | |
| 3 F | TATCAGATGGAGGATGTTCAC | 3′ terminus of phage C11 genome |
| 3 R | GCTGAGAGAGACCTTGTTCC | |
| 5 F | GGTGTTCCATCCACTCCCTG | 5′ terminus of phage C11 genome |
| 5 R | GTTCGCCTTCTGCCAGTTAT | |
| S1 | AGCCTGCTCTCTCCGTTC | The DNA probe for Southern blot analysis |
| S2 | TCTCAAGAGTGCTGTCCC | |
| SM-F | ACACTGAAAACCTTGACTCC | Amplification of the fragment with a mutation generating a EcoRI site |
| S2 | TCTCAAGAGTGCTGTCCC |
*The bases underlined represent the added restriction enzyme sites.