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vimE can be cotranscribed with vimA or Pg0792. One microgram of total DNase-treated RNA of P. gingivalis W83 from exponential phase (OD600 of 0.6) using a RiboPure kit (Ambion) was subjected to RT-PCR. (A) Cotranscription of vimA and vimE. Lane 1, P7 and P3 plus reverse transcriptase; lane 2, P7 and P3 minus reverse transcriptase. (B) Cotranscription of vimE and Pg0792. Lane 3, P1 to P5 plus reverse transcriptase; lane 4, P1 to P5 minus reverse transcriptase.
