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. 2004 Oct;72(10):5555–5564. doi: 10.1128/IAI.72.10.5555-5564.2004

FIG. 9.

FIG. 9.

The Rgp active site is not labeled by DNS-EGR-CK in P. gingivalis FLL93 extracellular fractions. Acetone-precipitated extracellular proteins from P. gingivalis W83, P. gingivalis FLL93, and P. gingivalis FLL92 were labeled with the fluorescent irreversible inhibitor DNS-EGR-CK and were subjected to SDS-PAGE on 4 to 12% NuPAGE gels (Invitrogen). The gels were viewed under UV light to visualize labeled proteins. (A) All the lanes were labeled with DNS-EGR-CK. (B) All the lanes were labeled with DNS-EGR-CK in the presence of 50 μM leupeptin. Each lane contains 20 μg of protein. The arrow indicates the DNS-EGR-CK-labeled protein band. E, exponential phase; L, stationary phase.