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. 2016 Dec 20;15:597. doi: 10.1186/s12936-016-1641-7

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© The Author(s) 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Primer selection analysis. a The frequency of each primer as ranked by the frequency of occurrence. The y-axis represents the calculated ratio of the frequency in the parasite (desired) genome against frequency in the human (contaminating) genome. The x-axis shows the order of ranking by the frequency of occurrence ratio. b DNA yield obtained following the selective whole genome amplification with different pools of probe sets. Probe_10 represent a pool of the first top 10 ranking primers. Probe_20 is a cumulative mixture consisting of all the Probe_10 primers plus the next 10 probes in that order. Probe_28 is a cumulative mixture of all the first 28 primers (Probe_10, Probe_20 and the next 8 probes in the order of frequency ranking)