Fig. 2.

The magnitude of SIVmac239 Env responses is dependent on the co-encoded gag sequence. CD1 mice were primed with Ad5 vaccines co-encoding SIVmac239 env with either SIVmac239 gag (AdSgSe) or HIV-1 CON B gag (AdHgSe) as illustrated in the experimental outline (a). Asterisk indicates time of serum harvest. (#) indicates harvest of spleens. 10 respectively 9 mice from each group were then boosted with MVA encoding SIVmac239 gag, pol, and env (MVAgpe) truncated at amino acid 733. Immunogenicity was investigated 52 and 69 days post priming immunization and also 10 days post boosting with MVA (d.69). b Co-encoding SIVmac239 gag compared to HIV-1 CON B gag with SIVmac239 env led to significantly increased end point titers against SIVmac239 Env, both after priming immunization, and also after the MVA boost. c The Avidity Index was determined in the sodium citrate displacement assay against SIVmac239 Env. d Neutralization titers against SIVsmE660.11 were determined at serum dilution starting at 1:500 (indicated with dotted line). b–d depicts individual mice with horizontal lines indicating mean with SEM (b) or geometric mean (c, d)