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. 2016 Jul 12;44(20):9942–9955. doi: 10.1093/nar/gkw631

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© The Author(s) 2016. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 6. — TRBP serines 283/286 are essential for the TRBP/S6K2 interaction in HDLECs. (A) PLA analysis of TRBP and S6K2 in HDLECs transduced with TRBP-WT (150 ml/80 000 cells), TRBP-AA (600 ml/80 000 cells), and TRBP-DD lentiviruses (200 ml/80 000 cells). The used volumes were calculated and verified to ensure equal TRBP protein expression between WT and mutant TRBP proteins. Nuclei (blue) and F-actin (green) staining are also shown (scale bar: 10 μm). PLA events (in red) are indicated with yellow arrows. B. Dot plot illustrating mean number of TRBP/S6K2 PLA events detected under the same conditions with (A). Values presented are means ± SD. P values are shown (*P < 0.05; ***P < 0.001). (C) Co-immunoprecipitation of S6K2 and TRBP in HEK293 cells over-expressing S6K2 and TRBP. Cells were transduced with S6K2 and TRBP-WT or TRBP-DD lentiviruses and harvested for immunoprecipitations when 80% confluent. (D) Quantification of co-immunoprecipitated TRBP with S6K2 in cells overexpressing TRBP-WT or TRBP-DD.