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. 2016 Jul 20;44(20):9650–9666. doi: 10.1093/nar/gkw642

Figure 6.

Figure 6.

Expression of omrA and omrB can be activated by non-phosphorylated OmpR. (A) The β-galactosidase activity of promoter fusions to omrA, omrB, ompC or ompF was measured in LB in exponentially growing cells with varying OmpR levels and/or phosphorylation. Activities are shown relative to that of wt cells. Strains used in this experiment are MG1690, MG1810, MG2043, MG2044 (PompF), MG1892, MG2018, MG2045, MG2046 (PompC), MG1004, MG1811, MG2039, MG2040 (PomrA), MG1005, MG1812, MG2041 and MG2042 (PomrB). The β-galactosidase activities of the different fusions in the wt strains were, in Miller units, 22 (PomrA fusion), 63 (PomrB), 11292 (PompC) and 3618 (PompF). (B) Activity of the same omrA, omrB and ompC transcriptional fusions was measured in wt cells or upon OmpR overproduction in ΔenvZ and ΔackA-pta background, both in omrAB+ or omrAB− strains. Strains are MG1892, MG2203, MG2046 and MG2206 (PompC fusion), MG1004, MG2201, MG2040 and MG2204 (PomrA), and MG1005, MG2202, MG2042 and MG2205 (PomrB).