Figure 1.

Estimation of normalization parameters. (A) Positive size factor estimated from NanoStringDiff against its true value; (B) Housekeeping size factor estimated from NanoStringDiff against its true value; (C) Background noise estimated from NanoStringDiff against its true value; (D) The overall size factor estimated from NanoStringDiff, DESeq2 and edgeR against its true value; For NanoStringDiff, the estimated overall size factor was the product of the estimated positive and housekeeping size factors; For DESeq2 and edgeR: the estimated overall size factor was directly calculated from the algorithm. Results were from a dataset simulated based on the Horbinski data with three replicates and averaged across the replicates.