Figure 5.

microRNA validation using Q-PCR. Total RNA used for NanoString was reversed transcribed to cDNA using specific miRNA primers from the TaqMan MicroRNA Assays and reagents from the TaqMan MicroRNA Reverse Transcription (RT) Kit. Individual miRNA expression levels were assessed by Q-PCR. Values were normalized to β-actin (top panel), 18S (middle panel) or U6 (bottom panel) as indicated and reported relative to GFP control. Experiments are depicted as the mean relative miRNA expression +/− standard deviation based on at least triplicate determinations. * indicates P < 0.05 based on a two-sample t-test.