Figure 3.

Impact of individual ARL sequence changes on PriA DNA unwinding in isolation and in the presence of SSB or PriB. Fraction of DNA unwound (synthetic nascent lagging strand unwound) measured after incubating 1.2 nM PriA variants with 1 nM 5′ ³²P-labeled synthetic DNA fork (depicted in upper inset and Supplementary Table S1 (structure #2, which is comprised of four oligonucleotides, with a 5 nt ssDNA gap between parental duplex and nascent lagging strand 5′ end). Top: PriA in isolation. Middle: PriA unwinding with DNA prebound by SSB (250 nM). Bottom: PriA unwinding with DNA prebound by PriB (10 nM). Data are mean of three replicates ± SD.