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Co-stabilization of AATF, NOL10 and NGDN. (A) HeLa cells were transfected with the indicated siRNAs. After 72 h, localization of various factors was analyzed by IF. AATF, NOL10 and NGDN were co-stained with FBL as a nucleolar marker. Scale bar, 20 μm. (B) Analysis of RNAi efficiency and protein levels by immunoblotting after 72 h of RNAi. Two independent siRNAs targeting AATF, NOL10 or NGDN and one siRNA for FBL depletion were used.
