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. 2016 Sep 5;44(20):9803–9820. doi: 10.1093/nar/gkw790

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© The Author(s) 2016. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 5. — Mapping of protein interaction domains between AATF, NOL10 and NGDN. (A) Scheme of protein domains and secondary structure predictions for AATF, NOL10 and NGDN. (B–D) StHA-tagged fl and truncated versions of AATF, NOL10 and NGDN were expressed by transient transfection in HEK293 cells for 48 h. GFP-StHA was used as negative control. StHA-tagged proteins were isolated using StrepTactin sepharose (Strep PD). Protein levels in the inputs (0.45%) and the retrieved protein complexes (30%) were analyzed by immunoblotting using the indicated antibodies.