Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Sep 19;44(20):9821–9830. doi: 10.1093/nar/gkw807

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2016. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

PMC Copyright notice

Figure 2. — AtPOT1a_OB1 specifically binds telomeric DNA. (A) EMSA assay results for POT1a_OB1-(TTTAGGG)₅ binding. 5′ labeled (TTTAGGG)₅ was titrated with increasing POT1a_OB1 (0-820 nM). (B) The K_d (app) of POT1a_OB1 for (TTTAGGG)₅ was determined as described in Materials and Methods using data from Figure 2A. (C) EMSA based competition assay results for POT1a_OB1-(TTTAGGG)₅ using a fixed POT1a_OB1 concentration (∼500 nM) and 50 to 100-fold excess of the nucleic acids specified. NS DNA stands for non-specific DNA (5′-TTAATTAACCCGGGGATCCGGCTTGATCAACGAATGATCC-3′ (ref. 32)). (D) Results of EMSA based displacement assay using ∼500 nM POT1a_OB1 in the absence and presence of 100- to 200-fold excess of different oligonucleotides to assess the specificity of POT1a_OB1 binding.