Figure 4.

Discrimination for processing of dGTP and nucleotide 1 employing purified KOD exo- DNA polymerase mutants; reactions were stopped after 5 min. A total of 5 nM DNA polymerase was used for processing of dGTP, 20 nM enzyme was employed for processing of nucleotide 1. Discrimination ratios were determined by calculating the quotient of % primer extension opposite C and % primer extension opposite 5mC. Discrimination ratios were color coded as indicated. Experiments were repeated four times in independent experiments, errors are given in the Supplementary Figure S4.