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. 2016 Sep 19;44(20):9881–9890. doi: 10.1093/nar/gkw812

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© The Author(s) 2016. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 6. — PAGE analysis of single-nucleotide incorporation primer extension experiments including thermostable inorganic pyrophosphatase. (A) Processing of dGTP by the KOD exo- wild type; (B) employing dGTP and KOD exo- G245D; (C) incorporation of nucleotide 2 by KOD exo- G245D. A total of 5 nM of the respective DNA polymerase were used for processing of dGTP; 20 nM for nucleotide 2, 100 μM dGTP/ dGT*P; 0.5 U/ml thermostable inorganic pyrophosphatase. Reactions were stopped after indicated time points. Results are from four independent experiments.