Table 1. Steady-state kinetic analysis of single nucleotide incorporation primer extension opposite C or 5mC by KOD exo- DNA polymerase wt and G245D.

dNTP	enzyme	template	KM [μM]	kcat [s−1]	kcat/KM [s−1 μM−1]
dGTP	wt	C	4.0 ± 0.3	5.9 ± 0.1	1.5 ± 0.1
		5mC	3.3 ± 0.3	3.5 ± 0.1	1.1 ± 0.6
dGTP	G245D	C	9.2 ± 2.2	7 ± 0.9	0.76 ± 0.1
		5mC	9.6 ± 1.9	2.7 ± 0.3	0.28 ± 0.41
2	G245D	C	344.0 ± 49.6	2.42 ± 0.22	0.0070 ± 0.0006
		5mC	203.8 ± 32.2	0.585 ± 0.043	0.0029 ± 0.0002

Ratios calculated by the quotient of k_cat (C)/k_cat(5mC) and k_cat/K_M (C) and k_cat/K_M (5mC), respectively.