MALAT1 RNA in liver, kidney, inner ear and brain at P1, P7, P15 and P30 after intra-amniotic cavity injection of ASO-MALAT1. (A) The average quantity of MALAT1 RNA normalized to β-actin RNA (ACTB) in indicated tissues from mice treated with 80 μg ASO-MALAT1 or ASO-C. Error bars represent s.e.m., *P < 0.05, **P < 0.01, one-tailed, two sample t-test. (B) Graphs depict the average relative quantity (RQ) of MALAT1 RNA normalized to ACTB shown relative to control samples in the liver, kidney, inner ear and brain from P1, P7, P15 and P30 mice subjected to intra-amniotic cavity injection of 80 μg of ASO-MALAT1 (n = 5, 6, 5, 5, respectively) or ASO-C (n = 3,3,4,4, respectively). The dotted lines represent the normalized control value of 1. Error bars represent s.e.m. **P < 0.01, ***P < 0.001 Kruskal–Wallis test with Dunn's Multiple Comparison test (kidney) or one-way ANOVA with Bonferroni's Multiple Comparison test (liver, inner ear, brain).