Figure 6.

Ush1c.216A splicing in the inner ear of P22 mice dosed with ASO-Ush by amniotic cavity injection at E13-13.5. (A) Semi-quantitative radiolabeled RT-PCR analysis with primer sets specific for human USH1C (to specifically amplify only the knocked in human portion of the Ush1c^216A allele (28)) and mouse Gapdh was performed on RNA isolated from the inner ear of P22 Ush1c^216AA or Ush1c^216GA mice treated with ASO-Ush at E13-13.5 by transuterine injection to the amniotic cavity (IA) (n = 3), or neonatal Ush1c^216AA mice treated at P5 with ASO-Ush by intraperitoneal (IP) injection (n = 3), or untreated P22 Ush1c^216AA (control)(n = 3) mice. (B) Quantification of correctly spliced mRNA from the Ush1c^216A allele. Error bars represent s.e.m (n = 3). *P ≤ 0.05, one-tailed, two-sample t-test.